MHC Class II (RT1D) 抗体
This anti- antibody is a 小鼠 单克隆 antibody detecting in FACS, IHC (fro) 和 IP. Suitable for 大鼠.
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Quick Overview for MHC Class II (RT1D) 抗体 (ABIN114389)
抗原
MHC Class II (RT1D)
适用
大鼠
宿主
小鼠
克隆类型
单克隆
标记
非结合性
应用范围
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)
克隆位点
OX-17
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纯化方法
- Protein G affinity chromatography
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免疫原
- Rat spleen membrane glycoproteins depleted of Ia-A antigens Immunocyte Donor: BALB/c spleen Fusion Partner: X63 Ag8.653
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亚型
- IgG1
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anti-MHC Class II (RT1D) antibody (Biotin)
适用: 大鼠 FACS, IHC (fro) 宿主: 小鼠 Monoclonal OX-17 Biotin
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应用备注
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Flow Cytometry: 1/250 - 1/500 (see Protocols). Immunhistochemistry. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
实验流程
- FLOW CYTOMETRY ANALYSIS: METHOD: 1. Prepare cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-Rat. cell separation medium. 2. Wash 2 times. 3. Resuspend cells to 1x10e6 cells in approximately 50 µl media A in a microcentrifugetube. (i. e. 50 µl of cells resuspended to 2x10e7 cells/ml). The contents of 1 tube represent 1test. 4. To each tube add 50 µl of a 1: 250 - 1: 500 dilution of this Ab. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (FITC Goat anti-mouse IgG (H+L)) at 1: 700 dilution. 9. Incubate tubes at 4°C for 30-60 minutes. (It is recommended that the tubes areprotected from light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C in Media B. 11. Resuspend the cell pellet in 50 µl ice cold Media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in phosphate buffered saline. (This stains dead cells by intercalatingDNA). MEDIA: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2 M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% bovine serum albumin + sodium azide (100 µlof 2 M sodium azide in 100 mls). RESULTS: Rat Strain: Lewis RatCell Concentration: 1x10e6 cells per testAntibody Concentration: 1: 400Isotypic Control: Mouse IgG1, κCELL SOURCE PERCENT STAININGThymus 12%Spleen 34%Lymph Node 25%STRAIN DISTRIBUTION: Antibody Concentration: 1: 100Strains Tested: Wistar, Buffalo, Brown Norway, Fischer 344Positive: Wistar, Buffalo, Brown Norway, ACI, Fischer 344Negative: none
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限制
- 仅限研究用
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浓度
- 1.0 mg/mL
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缓冲液
- PBS with 0.02 % sodium azide
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- MHC Class II (RT1D)
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别名
- MHC Class II RT1D
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背景
- Synonyms: HLA Class II
抗原
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